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Electron microscopes

Version:2018.10.19  
一、 Electron microscope would just use by the microscope technician.
二、 Reservation Precautions:
  1.

Please reserve by the CGMH service reservation system(Operation manual is on the right side.)After

print the Requisition,come to the microscope lab 2.We will arrange the  service date after

sample's process.Be aware that if your proposal is approved by CGMH,you don't need to stamp,and

if not,you need proposal director's stamp and sign.

  2.

Please check your sample's condition that it is suitable with our facility.We will charge no matter

what happened uneless facility malfunction. 

  3.

The time for our facility is hourly rate.After finish the service,we will burn the data to the

CD/DVD.We can't use USB flash drive.If your service has finished before you originally reserve,we

still charge based on your reservation on the system.Please note that if you over 30 minutes more

than you reserve, you need to charge a hour.

  4.

If you would like to cancel the reservation,please do it within 24 hours before your reservation

start.We still charge if you don't. 

  5.

Microscopy Core laboratory provide CD/DVD for users.User's data will save for 3 month.We will

organize these irregularly.

三、 Term of use:
  Sample's conditon and requirement for electron microscopy
  1 Precaution for dealing with sample:
    i. Sample:
      A. Please come to our lab and get the fixation buffer.
      B. Please let the sample in the fixation buffer at 4℃ and soon deliver to our lab.
      C. Please pay attention to the directivity of the tissue.
    ii. Sample's format:
      A. Tissue:
        a. TEM Sample:Size of the tissue is smaller than 2 x 2 x 2 mm3.
        b. SEM Sample:Size of the tissue is smaller than 10 x 10 x 2 mm3.
        c.

As mentioned above, if your smaple is cornea and retina( including choroid and sclera),

we need to check the directivity and the problem about retinal detachment.The thickness

of the sample should be smaller than 2mm.

      B. Cell:More than 106 cells。
      C. Prepare SEM Sample by yourself:
        a.

Sample's plane length and width should be smaller than diameter 2cm.Height should be

higher than 2cm.

        b.

Sample's section length and width should be smaller than diameter 2cm.Height should be

higher than 1cm.

        c. Sample should be dry and non-voc volatile.
        d. We reject the toxic,caustic,explosive,radioactive and magnetic sample and specimen.
      D.

Prepare SEM Sample by yourself:The diameter of sample is 3mm and thickness is 0.1mm.Thin

area and the slice thickness is nearly 90mm.

四、

When you publishes your paper,we hope you would mention "The authors would like to thank for technical

assistance of this reasearch.The Microscopy Core Laboratory, Chang Gung Memorial Hospital, Linkou is

acknowledged for microscopy analysis." in the acknowledgment part. The services of our center are mainly

academic research.When you reserve the services listed in the center, please describe in the acknowledgment

part of the article when the paper are published. This will be greatly help to us in applying for funding

and maintaining the quality of our services.Please send us a copy of the paper or tell us the volume,number

of pages or title of the published paper,We are so grateful for your kindness!